I really like Hank Green’s Crash Course biology videos! I recommend going over some theory and getting out in the world and applying your knowledge.
If you’re interested in microscopy you can find paper microscopes on amazon. A good introductory microscopy exercise be to grab some dirt or pond water and try to identify some microorganisms.
Excuse formatting, I’m on mobile!
I'll agree with you that it's possible those could be bubbles or some kind of artifact. However, those other pictures of C. elegans that you mentioned don't really show the germline very well either. Hermaphrodite germlines look like this and those weird bubbles kind of line up along where the mitotic zone and rachis are located.
I also agree, it's a great picture. I'm always a fan of great worm images!
Nothing that complicated. This baby puts the picture right on to the computer screen. I take it off the stand and slide it along the glass with the finger on the zoom wheel or use a sucker and stick it to the glass when there's something I want to film longer than I am willing to hold still lol:
see also https://en.wikipedia.org/wiki/Structural_coloration
if you wanted to be sure you'd maybe test it with "empirically" with a physically based renderer. i dont think anything stops you from throwing a model of a ton of microscopic stuff in there if your computer can handle it.
>To stabilize the emulsion you have to add a surfactant, in this case a few drops of soap.
I do that frequently to make water-based solutions spread out evenly on a slide. Normally, the water globs up due to surface tension, but adding a little wetting agent breaks that up.
I use Kodak Photo-Flo, which is less "soapy" than regular soap.
I presume you know that you need TWO polarizers to do polarized light microscopy? The sample has to sit between them.
A proper retarder is a carefully-constructed filter with precise properties, but if all you want is the psychedelic colors, you can just get cheap mica sheets from a craft store. This is what I am using for most of my shots, although I also found some cheapish precision retarders at Edmund Optical.
The layout from top to bottom you want is:
Camera
Good-quality polarizer
Microscope lens
Sample
Mica slice
Second polarizer
Light source
If you rotate the mica and bottom polarizer, either together or independently, you will get very different effects.
Amazon. It was a pack of 'craft materials' I don't know how it is used in arts and crafts, but it works well as a polarising retarder...
https://www.amazon.co.uk/gp/product/B00161U8B8/ref=ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1
RMS thread to Nikon adapter on Amazon is $25.
You can also find all kinds of cheap stuff like that on eBay.
The StackShot rail is great, but you can also do this manually, which I do with my other microscopes. Take a shot, goose the focus, take a shot, goose the focus,...
OMAX 40x-2000x compound microscope (https://www.amazon.com/gp/product/B0094JTZOU/) I really like and it's a solid microscope that's comparatively cheap. I took the photo just by holding my phone up to one of the binocular eyepieces and holding it steady.